The setting of this game was based on two areas: Limassol and Kyrenia. The look of the area is very similar to some of the cities in the first game, going as far as having the same atmosphere. Due to the small setting, Horseback riding was removed in Bloodlines. Like in the previous game, Altaïr can synchronize from high vantage points around the city in order to map out the city. Although the map itself is already drawn out, synchronizing will provide the locations of various side-missions.
New assassination techniques were featured in Bloodlines. Weapons from the previous game return in Bloodlines, such as Altaïr's sword, his short blade, throwing knives, and his fists. His usage of each of the weapons is very similar to the first Assassin's Creed. Assassinations are also similar to the first game, although the animation has been toned down to better fit the system. A new, yet very minor assassination technique has been added to the game, allowing Altaïr to pull opponents off of ledges, similar to Assassin's Creed II.
The 40th Day focuses on two-player cooperative play and employs a cover system. It features Tyson Rios and Elliot Salem, the two protagonists from the original game, as combatant partners who, with the assistance of their handler Alice Murray, must fight to survive and prevail over invading forces that have engulfed Shanghai, China in a devastating terrorist attack.